目的 :探讨细胞周期调控蛋白 P2 1CIP1 / WAF1 和细胞粘附分子 CD44 V6 在恶性卵巢上皮性肿瘤中的增殖、浸润、转移过程中的作用及相互关系。方法 :采用免疫组化方法 (im munohistochemical assay IHCA)检测 5 5例恶性卵巢上皮性肿瘤组织中 P2 1CIP1 / WAF1 蛋白和 CD44 V6 的表达。结果 :(1)恶性卵巢上皮性肿瘤中 P2 1CIP1 / WAF1总体阳性率为 5 5 .5 4% ,其中 期的阳性率为 2 1.43%低于 至 期的阳性率 6 5 .85 % (P<0 .0 5 ) ,伴有淋巴结转移的原发灶中的阳性率 31.5 7%明显低于无淋巴结转移的原发灶中的阳性率 6 6 .6 7% (P<0 .0 5 )。 (2 ) CD44 V6 在 到 期癌组织中表达阳性率差别无显著性 (P>0 .0 5 ) ,而在 、 期中强阳性 ( 、 )率 5 3.13%明显高于 、 期 13.0 4%(P<0 .0 5 ) ,在有淋巴结转移的原发灶中的强阳性率 (6 8.42 % )高于无淋巴结转移组 19.44 % (P<0 .0 1)。 (3) P2 1CIP1/ WAF1与 CD44 V6 在有淋巴结转移的原发灶中的表达呈负相关 (P<0 .0 5 )。结论 :P2 1CIP1 / WAF1 抑制恶性卵巢上皮性肿瘤增殖、浸润、转移 ,而 CD44 V6 作用相反 OBJECTIVE: To investigate the roles and relationships of the cell cycle regulatory proteins P2 1CIP1 / WAF1 and cell adhesion molecule CD44 V6 in the proliferation, invasion and metastasis of malignant ovarian epithelial neoplasms. Methods: The expressions of P2 1CIP1 / WAF1 protein and CD44 V6 in 55 malignant ovarian epithelial tumors were detected by immunohistochemistry (IHM). Results: (1) The overall positive rate of P2 1CIP1 / WAF1 in malignant ovarian epithelial tumors was 55.44%, with the positive rate of 2 1.43% in the mid-stage was lower than the positive rate of 65.85% (P < 0.05). The positive rate in the primary tumor with lymph node metastasis was 31.5 7%, which was significantly lower than that in the primary tumor without lymph node metastasis (66.76%, P <0.05). (2) There was no significant difference in the positive expression rate of CD44 V6 in cancerous tissues (P> 0.05), while the positive rate of CD44 V6 in the mature period was 53.13% <0.05). The positive rate in the primary tumor with lymph node metastasis (6 8.42%) was higher than that in the non-lymph node metastasis group (19.44%, P <0.01). (3) The expression of P2CIP1 / WAF1 and CD44 V6 in the primary tumor with lymph node metastasis was negatively correlated (P <0.05). Conclusions: P2CIP1 / WAF1 inhibits the proliferation, invasion and metastasis of malignant ovarian epithelial tumor, while the effect of CD44 V6 is opposite