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为研究老年脑梗塞患者外周血淋巴细胞热休克蛋白 70 (HSP70 )的变化规律、变化程度及其与病情轻重、梗塞部位、预后的关系 ,将 6 5例 6 0岁以上老年脑梗塞患者分为轻、中、重型三组 ;选择 34例健康老年人作为对照组。应用Westernblot法测定其外周血淋巴细胞中HSP70表达水平。结果显示 ,在起病后 2 4小时内 ,轻、中、重型HSP70表达水平分别为 (70 8 6 7± 193 5 6 )、(5 85 95± 16 5 39)及 (42 1 18± 12 3 87)积分光密度 ,各组之间比较及其与健康对照组的 (86 0 8± 34 13)积分光密度比较差异有显著性 (P <0 0 5 )。随病程延长 ,HSP70表达水平逐渐下降 ,轻型脑梗塞组下降幅度较大而重型脑梗塞组下降幅度较小 (P <0 0 1)。脑梗塞的部位与HSP70表达水平无相关关系。结果提示 ,测定老年脑梗塞患者起病后 2 4小时内HSP70表达水平 ,可为临床早期诊断脑梗塞、估计病情轻重、判断患者预后提供参考
To study the changes of HSP70 and its relationship with severity of disease, infarction location and prognosis in elderly patients with cerebral infarction, 65 elderly patients with cerebral infarction over 60 years old were divided into Light, medium and heavy three groups; choose 34 healthy elderly as a control group. Western blotting was used to determine the expression of HSP70 in peripheral blood lymphocytes. The results showed that within 24 hours after onset, the expression levels of mild, moderate and severe HSP70 were (70 8 6 7 ± 193 5 6), (5 85 95 ± 16 5 39) and (42 1 18 ± 123 87), and there was significant difference between the integral optical density (86 0 8 ± 34 13) and the integral optical density of the control group (P 0 05). With prolonged course, the expression level of HSP70 gradually decreased, the degree of decrease in the light cerebral infarction group was larger and that in the severe cerebral infarction group decreased less (P <0.01). There was no correlation between the location of cerebral infarction and the expression of HSP70. The results suggest that determination of HSP70 expression in 24 hours after the onset of senile cerebral infarction patients for early clinical diagnosis of cerebral infarction, estimated severity of illness, to determine the prognosis of patients with reference