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利用改进的整体透明技术,以观察多胚性的亚麻受精后的胚珠,同时对整体透明方法进行了讨论。方法:利用60°C 1M氢氧化钠溶液处理植物材料,然后利用10%次氯酸钠溶液透明,于显微镜下进行解剖观察。结果:在显微镜下,能观察到亚麻受精后胚珠内的幼胚,通过解剖还能清楚地观察到胚珠内的双胚的排列方式;并确定供试材料的双胚的发生率为8%。讨论:与传统的整体透明和切片方法相比,本改进的整体透明方法省时省力,并且能够完成整体透明和解剖的3D数据采集,适用于多胚亚麻幼胚的观察。缺点是用DAPI染色DNA效果不好。
The use of improved bulk opacity techniques to visualize ovules after polyembryony flax insemination while the overall opacity method is discussed. Methods: Plant material was treated with 60 ° C 1 M sodium hydroxide solution and then dissected under a microscope using 10% sodium hypochlorite solution. Results: Under the microscope, the immature embryos in the ovules after flax fertilization were observed. The arrangement of the double embryos in the ovules was clearly observed by dissection. The incidence of double embryos in the test materials was 8%. DISCUSSION: Compared with the traditional method of overall transparence and slicing, this improved holistic and transparent method can save time and effort, and can achieve the overall transparent and anatomical 3D data collection, which is suitable for the observation of polyembryonic flax embryos. The disadvantage is that staining DNA with DAPI does not work well.