【摘 要】
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目的研究粘着斑激酶磷酸化在细胞外基质成份诱导平滑肌细胞粘附和迁移中的作用.方法通过纤粘连蛋白(fibronectin, FN)诱导培养的平滑肌细胞粘附迁移,以免疫沉淀和Western blo
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目的研究粘着斑激酶磷酸化在细胞外基质成份诱导平滑肌细胞粘附和迁移中的作用.方法通过纤粘连蛋白(fibronectin, FN)诱导培养的平滑肌细胞粘附迁移,以免疫沉淀和Western blot方法检测粘着斑激酶(focal adhesion kinase, FAK)及其磷酸化的表达量.将FAK反义寡核苷酸(antisense oligodeoxynucleotides, ODNs)经脂质体转染细胞,观察对FAK磷酸化、细胞粘附铺展和迁移的影响.结果 FN在显著诱导平滑肌细胞粘附和迁移时,FAK也呈明显表达,20?μg/ml FN可使其磷酸化处于较高表达量.脂质体可有效地介导ODNs转染,转染效率为86.7%±4.5%,FAK磷酸化表达量明显减少,5-60?μg/ml不同浓度FN组,细胞铺展率减少17.89%-27.67%,10、 20、 4 0和60?μg/ml FN组迁移细胞数也分别显著减少23.26%、21.63%、19.31%、17.88%(P
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