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目的:建立人胸腺重症联合免疫缺陷近交系(SCID)小鼠嵌合体模型并用此研究人NKT细胞的发育和分化及其抗EB病毒作用。方法:将人类胚胎胸腺细胞移植到SCID小鼠的胸腺建立人胸腺SCID小鼠嵌合体。用EB病毒感染已建立的人胸腺SCID小鼠嵌合体。检测嵌合体小鼠体内NKT细胞及EBV特异性NKT细胞的变化。结果:EB病毒有效的促进了NKT细胞以及EBV特异性CD8+NKT细胞的增殖,EBV感染嵌合体小鼠4周以后,每106嵌合体的胸腺细胞中可检测到3 000-3 500个EBV特异性NKT细胞,并且其中90%以上为CD8+。结论:成功建立人胸腺SCID小鼠嵌合体模型,并运用此模型发现人类EB病毒能特异性刺激NKT细胞增殖并且确定EBV特异性NKT细胞的存在。
OBJECTIVE: To establish a chimeric model of human thymic severe combined immunodeficient inbred (SCID) mice and to study the development and differentiation of human NKT cells and its anti-EB virus effect. Methods: Human thymus SCID mouse chimera was established by transplanting human embryonic thymocytes into the thymus of SCID mice. Established human thymus SCID mouse chimera with Epstein-Barr virus. To detect the changes of NKT cells and EBV-specific NKT cells in chimeric mice. Results: Epstein-Barr virus effectively promoted the proliferation of NKT cells and EBV-specific CD8 + NKT cells. After 4 weeks of EBV infection in chimeric mice, 3 000-3 500 EBVs were detected per 106 chimera thymocytes NKT cells, and more than 90% of them are CD8 +. Conclusion: The chimera model of human thymus SCID mice was successfully established. Using this model, we found that human Epstein-Barr virus can specifically stimulate the proliferation of NKT cells and determine the existence of EBV-specific NKT cells.