为了探讨脑缺血后血小板活化因子（ｐｌａｔｅｌｅｔ－ａｃｔｉｖａｔｉｎｇｆａｃｔｏｒ，ＰＡＦ）含量的变化，采用沙土鼠单侧脑缺血和大鼠局部脑缺血模型，用薄层层析（ＴＬＣ）结合生物法测定了缺血后脑组织ＰＡＦ的含量结果：（１）沙土鼠右侧颈总动脉结扎３ｈ再灌２ｈ，右侧大脑组织ＰＡＦ含量较左侧明显升高，分别为（４４０±０９０）和（１３０±０４２）ｐｇ／ｇ（Ｐ＜００５）；（２）大鼠左侧大脑中动脉（ＭＣＡ）凝闭后４８ｈ，左侧大脑组织ＰＡＦ含量右侧亦明显升高，分别为（５７４±０７４）和（０７６±０２４）ｐｇ／ｇ（Ｐ＜００５）据此分析了ＰＡＦ在脑缺血继发神经元损伤发病机制中的可能作用 In order to investigate the changes of platelet-activating factor (PAF) after cerebral ischemia, we used unilateral cerebral ischemia model of gerbil and rat model of focal cerebral ischemia, determined by TLC and bioassay The content of PAF in ischemic brain tissue  Results: (1) The right common carotid artery was ligated for 3h and then for 2h in right gerbil. The content of PAF in the right cerebral tissue was significantly higher than the left (440 ± 090) And (130 ± 042) pg / g (P <005). (2) 48 h after left middle cerebral artery (MCA) (574 ± 074) and (076 ± 024) pg / g (P <005), respectively. Based on this, we analyzed the role of PAF in the pathogenesis of neuronal injury secondary to cerebral ischemia Possible role