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Upon addition of Tb~(3+) to 16 nucleotides and homopolynucleotides, all of them showeda characteristic green emission from Tb~(3+), but with much different intensity, upon excitation inthe aromatic region of bases. The result suggested that nucleotides with at least one carbonylgroup in nucleotide bases are better enhancers to the fluorescence of Tb3+. The complexes of ATP,GDP and GTP with T5~(3+) are synthesized as two types of models. Guanine tpye nucleotides withone carbonyl group in the bases are the best enhancers, while adenine type nucleotides with nocarbonyl group in the bases are poorest enhancers to the fluorescence of Tb~(3+). Comparing theIR spectra of ATP, GTP, GDP and their Tb~(3+) complexes suggested that C-6 carbonyl group inGTP and GDP may be involved in complex formation, which may be responsible for the effectiveenergy transfer. This is further supported by comparing the UV spectra of ATP, Poly(A), GTP,and Poly(G) with their Tb~(3+) complexes in water solution.
Upon addition of Tb ~ (3+) to 16 nucleotides and homopolynucleotides, all of them showeda characteristic green emission from Tb ~ (3+), but with much different intensity, upon excitation inthe aromatic region of bases. The result suggested that nucleotides with At least one carbonyl group in nucleotide bases are better enhancers to the fluorescence of Tb3 +. The complexes of ATP, GDP and GTP with T5 ~ (3+) are synthesized as two types of models. Guanine tpye nucleotides withone carbonyl group in the bases are the best enhancers, while adenine type nucleotides with nocarbonyl group in the bases are poorest enhancers to the fluorescence of Tb ~ (3+). Comparing theIR spectra of ATP, GTP, GDP and their Tb ~ (3+) complexes suggested that C-6 This is further supported by comparing the UV spectra of ATP, Poly (A), GTP, and Poly (G) with their Tb ~ ( 3+) complexes in water solution.