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参茸鹿胎丸是由干漆等二十一味中药材,经加工制成。用于治疗脑血栓,脑瘀血后遗症,肢体偏瘫,手足麻木,语言障碍等。本文以方中细贵药材红参为其定量指标,采用高效液相色谱法,选用十八烷基硅烷键合硅胶为填充剂;以乙腈-0.1%磷酸(21:79)为流动相;检测波长为203nm,流速:1.000ml/min,理论塔板数按人参皂苷Rg1峰计算应不得低于2500。其人参皂苷Rg1的标准曲线:Y=19.7448+174.8048xr=0.9997,由此确定人参皂苷Rg1在0.250～6.250μg范围内呈良好线性,平均回收率(n=5)人参皂苷Rg199.26%,RSD2.2%。表明该方法灵敏度高,重现性好,结果准确、可靠。
Ginseng deer fetal pill is dry paint, such as twenty-one Chinese herbal medicines, processed. For the treatment of cerebral thrombosis, brain blood stasis sequelae, limb hemiplegia, numbness of hands and feet, language barriers. In this paper, the square herbs red ginseng as its quantitative indicators, using high performance liquid chromatography, octadecylsilane bonded silica as a filler; acetonitrile -0.1% phosphoric acid (21:79) as the mobile phase; detection Wavelength of 203nm, flow rate: 1.000ml / min, theoretical plate number calculated by ginsenoside Rg1 peak should not be less than 2500. The standard curve of the ginsenoside Rg1: Y = 19.7448 + 174.8048xr = 0.9997, which confirmed that ginsenoside Rg1 showed a good linearity in the range of 0.250-6.250μg with an average recovery (n = 5) of ginsenoside Rg199.26% and RSD2 .2%. It shows that this method has high sensitivity, good reproducibility and accurate and reliable results.