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The production of malting barley in China can’t meet the demand of beer industries because of poor quality and it becomes a bottleneck problem in beer manufacture industry. In this paper, TrxS gene cloned from Phalaris coerulescens was transferred into barley cultivar Yupi 1 (YP1)via biolistic bombardment. 1206 immature embryos were bombarded and seven transgenic plants carrying TrxS gene were confirmed by PCR and PCR-South blotting analysis. TrxS gene was expressed in transgenic plants by RT-PCR analysis. The activity of Trxh and αt-amylase of transgenic line were higher than that of non-transgenic line, which is helpful to improve malting quality of barley.