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本文介绍了用二种方法即液体滤过法和同位素廓清法对狗活体牙上所制备的洞型作渗透性追踪测定。作者用体重为20—26kg的8条狗作为实验对象,在全麻下制备两侧下颌第一磨牙颊面洞型,洞深及牙本质层,酸处理后,将事先制作好的圆锥形塑料小室置于窝洞内,并严密封闭,务使小室与牙体之间做到无泄漏。小室内注满含有0.02％荧光素的磷酸盐缓冲液(PBS),在外压为200cm/H_2O,液体从小室移入牙齿的情况下完成所有的测定工作。实验
This article describes the two methods of liquid filtration and isotope decontamination of the dog's teeth on the prepared hole for tracking the permeability of the track. The authors used eight dogs weighing 20-26 kg as experimental subjects to prepare buccal cavity of the first molar on both sides under general anesthesia, deep hole and dentin layer. After the acid treatment, the preformed conical plastic Small room placed in the nest hole, and closed, so that between the chamber and the tooth so that no leakage. The chamber was filled with phosphate buffer (PBS) containing 0.02% fluorescein and all the assay was done with an external pressure of 200 cm / H 2 O, with the liquid moving from the chamber to the tooth. experiment