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目的探讨人疱疹病毒６型（ＨＨＶ－６）感染的免疫发病机理。方法用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）和双抗体夹心酶联免疫吸附试验（ＥＬＩＳＡ）检测白细胞介素－１０（ＩＬ－１０）等细胞因子的产生。结果ＨＨＶ－６诱导单核细胞表达和产生ＩＬ－１０，细胞因子ｍＲＮＡ动力学研究发现ＴＮＦ－α（肿瘤坏死因子）、ＩＬ－１β及ＩＬ－６随ＩＬ－１０ｍＲＮＡ积累而减少，用抗人ＩＬ－１０单克隆抗体阻断ＨＨＶ－６诱导的内源性ＩＬ－１０，ＴＮＦ－α、ＩＬ－１β和ＩＬ－６ｍＲＮＡ的表达和因子产生明显增加，表明ＨＨＶ－６诱导的内源性ＩＬ－１０在转录水平能抑制单核细胞因子产生。结论ＩＬ－１０具有抑制Ｉ类辅助性Ｔ细胞（Ｔｈ１）反应、下调单核巨噬细胞功能等多种生物作用，推测ＨＨＶ－６诱导产生的内源性ＩＬ－１０与该病毒长期潜伏感染及其参予的免疫功能紊乱有关。
Objective To investigate the pathogenesis of human herpesvirus type 6 (HHV-6) infection. Methods The production of IL-10 and other cytokines was detected by RT-PCR and ELISA. Results HHV-6 induced monocyte expression and production of IL-10. The cytokine mRNA kinetics showed that TNF-α (tumor necrosis factor), IL-1β and IL-6 decreased with the accumulation of IL- -10 monoclonal antibody blocked the HHV-6-induced increase in the expression and production of endogenous IL-10, TNF-α, IL-1β and IL-6 mRNA, indicating that HHV-6 induces endogenous IL- Monocyte cytokine production can be inhibited at the transcriptional level. Conclusions IL-10 has multiple biological effects, such as inhibiting Th1 type I response and down-regulating the function of monocyte-macrophage cells. It is speculated that IL-10 induced by HHV-6 and long-term latent infection of the virus And its participation in immune dysfunction.