“微生物的实验室培养”是进行微生物研究和利用的基础,其基本操作技术是高中学生必备的技能。因此,“微生物的实验室培养”实验是高中选修教材中一个非常重要的实验。笔者从教学安排到实验操作等方面提出开设该实验的建议及怎样组织实验。一、实验教学内容分析“微生物的实验室培养”是选修1的专题2“微生物的培养与应用”中的第1个课题,它要求在传统发酵技术的应用的基础上,进一步全面深入学习微生物培养的基础知识和基本操作技术,同时也为微生物应用奠定基础,具有承上启下的重要作用。在生物技术模块中是学生应该选择的一个重要的操作实验,但由于该课题实验难度较大,多数中学实验器材不足、经费紧张等原因,在选择课题时都放弃该专题中的这个 “Microbial laboratory culture ” is the basis for microbial research and utilization, and its basic operating skills are essential skills for high school students. Therefore, the “Microbial Laboratory Culture” experiment is a very important experiment in high school elective materials. The author proposes to set up the experiment and how to organize the experiment from the teaching arrangement to the experimental operation. First, the content analysis of experimental teaching “laboratory culture of microorganisms ” is a subject 1 of the elective 1 “microbial culture and application ” in the first issue, which requires the application of traditional fermentation technology based on further Comprehensive and in-depth study of the basic knowledge of microbial culture and basic operating techniques, but also lay the foundation for the application of microorganisms, with the important role of enlightenment. In the biotechnology module, students should choose an important operation experiment, but due to the difficulty of the subject experiment, most of the high school experimental equipment is not enough, funding constraints and other reasons, in the choice of the subject to give up the topic in this