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目的 :阐明小茴香提取物对大鼠脂质过氧化的干预作用,初步证实小茴香对大鼠肝纤维化的药理影响。方法 :取100只实验大鼠,随机将其中15只大鼠设为空白对照组,将其余大鼠用复合因素法复制大鼠肝纤维化模型,并将其分为小茴香提取液低剂量组、小茴香提取液中剂量组、小茴香提取液高剂量组和模型对照组。用甲醇萃取法提取小茴香提取液,并分别以0.54、1.08、2.16g/kg的剂量对各小茴香提取液组大鼠进行干预,每日灌胃给药一次,用药8周。结果 :与正常对照组大鼠相比,模型对照组大鼠的SOD活性出现极显著下降(P<0.01),其MDA和HYP的含量出现极显著升高(P<0.01)。小茴香提取液低、中、高剂量组大鼠肝脏中SOD的活性与模型对照组大鼠相比均显著升高(P<0.05),小茴香提取液高剂量组大鼠肝脏中SOD的活性与模型对照组大鼠相比极显著升高(P<0.01)。小茴香提取液低、中、高剂量组大鼠肝脏中MDA的含量和HYP的含量与模型对照组大鼠相比均显著降低(P<0.05)。结论:小茴香有良好的抗肝脂质过氧化作用,可对抗肝纤维化。
Objective: To clarify the intervention of fennel extract on lipid peroxidation in rats, and to initially confirm the pharmacological effects of fennel on liver fibrosis in rats. Methods: 100 experimental rats were randomly selected. Fifteen of them were randomized into a blank control group. The other rats were used to replicate the model of hepatic fibrosis by compound factor, and divided into low dose group of fennel extract , Fennel extract medium dose group, fennel extract high dose group and model control group. The extract of fennel was extracted by methanol extraction, and each fennel extract group rats were intervened by 0.54, 1.08 and 2.16g / kg respectively. The rats were given gavage once daily for 8 weeks. Results: Compared with the normal control group, the SOD activity of the model control group showed a significant decrease (P <0.01), and the content of MDA and HYP significantly increased (P <0.01). The activities of SOD in the livers of low, medium and high dose fennel extract groups were significantly higher than those in the model control group (P <0.05). The activity of SOD in the liver of high dose fennel extract group Compared with the model control group, the expression was significantly increased (P <0.01). The contents of MDA and HYP in the liver of low, medium and high dose fennel extract groups were significantly lower than those in model control group (P <0.05). Conclusion: Fennel has a good anti-liver lipid peroxidation, can fight liver fibrosis.