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本研究采用RT-PCR技术从低钾胁迫的甘蔗根系中克隆得到一个长度为1 749 bp的与CBL互作的丝氨酸/苏氨酸蛋白激酶基因,命名为Ss CIPK23(Gen Bank登录号为KM981737),包含1 350 bp的开放阅读框(ORF),可编码一个449氨基酸残基的多肽。Ss CIPK23具有CIPK基因家族典型的蛋白激酶结构域和NAF调控结构域。进化树分析显示Ss CIPK23与其他作物的CIPK23关系较近,具有较高的保守性。q PCR分析结果表明,在低钾、干旱和盐胁迫下,Ss CIPK23的表达都发生改变。在低钾胁迫24 h后Ss CIPK23相对表达量开始上升,而且随着胁迫时间的延长,相对表达量持续增加;而在干旱和盐胁迫下,相对表达量最高出现在48 h,随后表达量开始下降,暗示了该基因在低钾、干旱和盐胁迫下发挥重要的调控作用,可为深入研究Ss CIPK23基因功能奠定坚实的基础。
In this study, a serine / threonine protein kinase gene with a length of 1 749 bp that interacted with CBL was cloned from low potassium stress sugarcane roots by RT-PCR and named Ss CIPK23 (Gen Bank Accession No. KM981737) , Contains a 1 350 bp open reading frame (ORF) that encodes a 449 amino acid residue polypeptide. Ss CIPK23 has a typical protein kinase domain and a NAF regulatory domain of the CIPK gene family. Phylogenetic tree analysis showed that Ss CIPK23 is more closely related to CIPK23 of other crops and has higher conservation. q PCR analysis showed that the expression of Ss CIPK23 changed under low potassium, drought and salt stress. The relative expression level of Ss CIPK23 began to increase after 24 h of low potassium stress, and the relative expression level continued to increase with the prolongation of stress time. Under drought and salt stress, the relative expression reached the peak at 48 h, Decreased, suggesting that the gene plays an important regulatory role under hypokalemia, drought and salt stress, which may lay a solid foundation for further study on the function of Ss CIPK23 gene.