目的：了解ＧＢＶＣ和ＨＣＶ的共同感染率，及不同引物和自制ＤＩＧ标记探针检测ＧＢＶＣ的效果。方法：定量ＲＴＰＣＲ检测肝炎患者血清标本中的ＨＣＶ，ＲＴｎｅｓｔｅｄＰＣＲ检测ＨＣＶＲＮＡ阳性标本中的ＧＢＶＣ，分析部分标本扩增产物的核苷酸和氨基酸序列，Ｓｏｕｔｈｅｒｎ杂交鉴定ＧＢＶＣ扩增产物的特异性。结果：２１１例丙型肝炎病人血清标本中，ＧＢＶＣ５’ＮＣＲ和ＧＢＶＣＮＳ３区检出率分别为３１．８％和２２．８％，总阳性率为３５．１％。部分标本扩增产物的核苷酸序列分析证实，ＲＴｎｅｓｔｅｄＰＣＲ获得的检测结果可靠，但序列中存在较高频率的随机突变。自制的ＧＢＶＣ５’ＮＣＲ和ＮＳ３ＤＩＧ标记探针有较高的特异性，可用于检测相应的扩增产物。结论：ＧＢＶＣ５’ＮＣＲ引物的检测效果优于ＧＢＶＣＮＳ３，ＧＢＶＣ与ＨＣＶ有较高的共同感染率。 Objective: To understand the common infection rate of GBV C and HCV, and different primers and homemade DIG labeled probe detection GBV C effect. METHODS: Quantitative RT-PCR was used to detect HCV in serum samples from hepatitis patients. RT-nested PCR was used to detect GBV-C in HCV RNA positive samples. Nucleotide and amino acid sequences of some samples were analyzed. Southern hybridization was used to identify GBVC amplification Product specificity. Results: The positive rates of GBVC5’NCR and GBVCNS3 in serum samples of 211 hepatitis C patients were 31.8% and 22.8% respectively, with a total positive rate of 35.1%. Nucleotide sequence analysis of the amplified products of some samples confirmed that RT -nestedPCR obtained reliable results, but there was a higher frequency of random mutation in the sequence. Homemade GBV  C5’NCR and NS3DIG labeled probe has a high specificity, can be used to detect the corresponding amplification products. Conclusion: The detection results of GBVC5’NCR primer is better than GBVCNS3, and GBVC and HCV have higher co-infection rates.