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目的:建立气相色谱-质谱联用法检测瑞芬太尼浓度。方法:采用液-液萃取法,用二氯甲烷提取人血浆瑞芬太尼,选用盐酸芬太尼作为内标定量,用气相色谱-质谱检测。结果:标准曲线在0.2~100μg.L-1范围内线性关系良好(r=0.9992),方法回收率为85%~92.5%,萃取回收率为98.1%~102.1%,日内RSD小于10.7%,日间RSD小11.8%。结论:本方法具有试剂成本低、准确度高、专一性好等优点,适用于临床瑞芬太尼血药浓度检测和药动学研究。
Objective: To establish a method for the determination of remifentanil by gas chromatography-mass spectrometry. Methods: Liquid-liquid extraction was used to extract human remifentanil from human plasma. Quantitative determination of fentanyl with fentanyl hydrochloride was carried out by gas chromatography-mass spectrometry. Results: The calibration curve showed good linearity (r = 0.9992) in the range of 0.2 ~ 100μg.L-1. The recoveries were in the range of 85% -92.5%. The recoveries were 98.1% -102.1%. The RSD was less than 10.7% Inter-RSD is 11.8% smaller. Conclusion: The method has the advantages of low reagent cost, high accuracy, good specificity and so on. It is suitable for the determination of clinical drug concentration and pharmacokinetics in remifentanil.