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目的分析多囊卵巢综合征(PCOS)患者的卵巢颗粒细胞(GCs)中载脂蛋白A-Ⅰ(ApoA-Ⅰ)的表达情况。方法收集接受体外授精-胚胎移植治疗的20例PCOS患者(PCOS组)和20例对照组妇女的GCs和术前检查的血清。采用Westernblotting技术检测GCs中ApoA-Ⅰ蛋白表达;采用Real-time PCR技术检测GCs中ApoA-ⅠmRNA表达;采用免疫比浊法检测血清ApoA-Ⅰ水平。结果 PCOS组GCs中ApoA-Ⅰ蛋白及mRNA的表达均显著低于对照组(P<0.001,P<0.01),PCOS组血清ApoA-Ⅰ水平亦显著低于对照组(P<0.05)。结论 PCOS患者GCs中ApoA-Ⅰ表达下调。
Objective To analyze the expression of apolipoprotein A-Ⅰ (ApoA-Ⅰ) in ovarian granulosa cells (GCs) from patients with polycystic ovary syndrome (PCOS). Methods Twenty GCOS patients and 20 preoperative serums from 20 PCOS patients (PCOS group) and 20 control women were enrolled in this study. Western blotting was used to detect the expression of ApoA-Ⅰ in GCs. Real-time PCR was used to detect the expression of ApoA-Ⅰ mRNA in GCs. ApoA-Ⅰ level was detected by immunoturbidimetry. Results The expression of ApoA-Ⅰprotein and mRNA in PCOS group was significantly lower than that in control group (P <0.001, P <0.01). The serum ApoA-Ⅰ level in PCOS group was also significantly lower than that in control group (P <0.05). Conclusions The expression of ApoA-Ⅰ in GCs is down-regulated in patients with PCOS.