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外源性ＧＭ３（１０ｎｍｏｌ／ｍＬ）、ＧＤ３（１ｎｍｏｌ／ｍＬ）可使ＳＭＭＣ－７７２１人肝癌培养细胞内钙浓度呈快速的短暂升高，其到达峰值时间为４５秒，一次作用后，内钙水平于２－３ｍｉｎ内恢复至对照水平。在一定时间间隔中连续几次加入ＧＭ３或ＧＤ３后内钙水平的变化表明，ＧＭ３所引起的［Ｃａ２＋］ｉ的增加依赖于内质网钙贮的释放和细胞外钙的流入；而ＧＤ３增加［Ｃａ２＋］ｉ与此二系统无关。进一步研究表明，在细胞内钙达峰值时，１０ｎｍｏｌ／ｍＬＧＭ３可使ＩＰ３（１，４，５）浓度增加９．３倍，ｃＡＭＰ浓度增加８２％；１ｎｍｏｌ／ｍＬＧＤ３反使Ｉｐ３浓度增加１．２倍，提示ＧＭ３、ＧＤ３升高内钙的不同机制。
Exogenous GM3 (10 nmol/mL) and GD3 (1 nmol/mL) can rapidly increase the intracellular calcium concentration of SMMC-7721 human hepatoma cells with a peak time of 45 seconds. After a single action, intracellular calcium levels Restore to control level within 2-3min. Changes in intracellular calcium levels after successive additions of GM3 or GD3 over a certain time interval suggest that the increase in [Ca2+]i induced by GM3 is dependent on the release of calcium stores from the endoplasmic reticulum and the influx of extracellular calcium; whereas GD3 increases [ Ca2+]i has nothing to do with this system. Further studies showed that at the peak of intracellular calcium, 10 nmol/mL GM3 increased the concentration of IP3 (1,4,5) by 9.3-fold, and the cAMP concentration increased by 82%; 1 nmol/mL GD3 reversed the Ip3 concentration by 1.2-fold. , suggesting that GM3, GD3 increase the different mechanisms of calcium.