本文介绍用2,3一二氨基萘(DAN)与食品中微量硒作用产生4,5-苯并苤硒脑的荧光测定法,并对实验条件进行了探讨。本方法的最低检出量为6.0ng,硒含量为0.01 ppm;0.1～1ppm时变异系数分别为9～13.3%、2.4～5.2%,回收率为90～105%。本法的精密度及准确度均高。 In this paper, the fluorescence determination of 4,5-benzodiazepine selenium with 2,3-diaminonaphthalene (DAN) and trace amounts of selenium in food was introduced, and the experimental conditions were discussed. The detection limit of this method was 6.0 ng and the selenium content was 0.01 ppm. The coefficients of variation were 0.1% ~ 1 ppm and 9 ~ 13.3%, respectively. The recoveries ranged from 90% to 105%. The precision and accuracy of this Law are high.