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目的探讨有氧运动联合抗阻力运动对糖尿病大鼠血管内皮功能及对磷脂酰肌醇-3激酶/蛋白激酶B/一氧化氮合酶(PI3K/Akt/eNOS)通路的影响。方法将SD大鼠随机分为对照组(NC)、糖尿病模型组(DM)、单纯有氧运动训练组(AE)、单纯抗阻力运动训练组(RE)及有氧运动+抗阻力运动训练组(ARE),检测各组内皮功能指标、PI3K/Akt/eNOS通路基因及蛋白表达情况。结果乙酰胆碱(Ach)浓度在3×10~(-5) mol/L时,ARE组舒张率高于AE组和RE组(P<0.05)。ARE组VEGF、一氧化氮(NO)表达量高于DM组[(21.45±3.48)vs(8.23±1.14)pg/ml,(25.67±3.19)vs(8.89±1.56)μmol/L,P<0.05]。ARE组PI3K、Akt及eNOS基因相对表达量高于DM组[(4.08±0.83)vs(0.27±0.11),(2.75±0.69)vs(0.26±0.09),(4.25±0.74)vs(0.59±0.19),P<0.05]。ARE组PI3K、磷酸化Akt(p-Akt)及磷酸化eNOS(p-eNOS)蛋白相对表达量高于DM组[(0.78±0.21)vs(0.20±0.09),(1.21±0.38)vs(0.54±0.19),(0.59±0.23)vs(0.16±0.08),P<0.05]。结论有氧运动联合抗阻力运动能改善糖尿病大鼠血管舒张功能,促进VEGF、NO的释放,作用与激活PI3K/Akt/eNOS信号通路有关。
Objective To investigate the effects of aerobic exercise combined with anti-resistance exercise on vascular endothelial function and the pathway of PI3K / Akt / eNOS in diabetic rats. Methods SD rats were randomly divided into control group (NC), diabetic model group (DM), aerobic exercise group (AE), simple anti-resistance exercise training group (RE) and aerobic exercise + anti-resistance exercise training group (ARE). The indexes of endothelial function and PI3K / Akt / eNOS pathway genes and protein expression in each group were detected. Results When the concentration of Ach was 3 × 10 ~ (-5) mol / L, the relaxation rate in ARE group was higher than that in AE group and RE group (P <0.05). The expression of VEGF and NO in ARE group was higher than that in DM group [(21.45 ± 3.48) vs (8.23 ± 1.14) pg / ml, (25.67 ± 3.19) vs (8.89 ± 1.56) μmol / L, P <0.05 ]. The relative expression of PI3K, Akt and eNOS in ARE group was significantly higher than that in DM group [(4.08 ± 0.83) vs (0.27 ± 0.11), (2.75 ± 0.69) vs (0.26 ± 0.09), (4.25 ± 0.74) vs (0.59 ± 0.19 ), P <0.05]. The relative expression of PI3K, p-Akt, and p-eNOS in ARE group was significantly higher than that in DM group [(0.78 ± 0.21) vs (0.20 ± 0.09), (1.21 ± 0.38) vs (0.54 ± 0.19), (0.59 ± 0.23) vs (0.16 ± 0.08), P <0.05]. Conclusion Aerobic exercise combined with anti-resistance exercise can improve the vasodilation function of diabetic rats and promote the release of VEGF and NO, which is related to the activation of PI3K / Akt / eNOS signaling pathway.