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目的探讨HSV-TK/GCV自杀基因系统对小鼠子宫颈癌细胞系Hela形成肿瘤的体内杀伤作用及其产生的旁观者效应。方法体内实验分Hela对照组、Hela/TK+Hela(1∶9)组、Hela/TK+Hela(1∶1)组和Hela/TK组。分别按实验组别要求接种3.0×106细胞/只于BALB/C小鼠的右腋窝皮下,观察各组肿瘤形成及肿瘤治疗情况。治疗结束后将标本制成石蜡切片和超薄切片进行病理学分析,RT-PCR检测HSV-TK基因在肿瘤组织中的表达情况。统计学分析采用SPSS软件进行完全随机的方差分析(ANOVA)。结果丙氧鸟苷(ganciclovir,GCV)可明显抑制Hela/TK细胞在BALB/C小鼠体内的肿瘤形成。经GCV治疗后,Hela/TK+Hela(1∶9)、Hela/TK+Hela(1∶1)组和Hela/TK组分别较对照组肿瘤体积缩小约11.1%、30.6%和47.2%;RT-PCR检测HSV-TK基因在肿瘤组织中有表达。实验组肿瘤组织与对照组相比存在明显的病理学改变。结论HSV-TK/GCV自杀基因系统在体内对子宫颈癌细胞有杀伤作用,且存在明显的旁观者效应。
Objective To investigate the in vivo killing effect of HSV-TK / GCV suicide gene system on mouse cervical cancer cell line Hela and its bystander effect. Methods In vivo experiments were divided into Hela control group, Hela / TK + Hela (1: 9) group, Hela / TK + Hela (1: 1) group and Hela / TK group. The mice were inoculated intraperitoneally with 3.0 × 10 6 cells / subcutaneous right axilla only in BALB / C mice according to the experimental group. The tumor formation and tumor treatment in each group were observed. After treatment, the specimens were made into paraffin sections and ultrathin sections for pathological analysis. The expression of HSV-TK gene in tumor tissues was detected by RT-PCR. Statistical Analysis Complete randomized analysis of variance (ANOVA) was performed using SPSS software. Results ganciclovir (GCV) significantly inhibited the tumorigenesis of Hela / TK cells in BALB / C mice. After GCV treatment, the tumor volume of Hela / TK + Hela (1: 9), Hela / TK + Hela (1: 1) and Hela / TK groups were reduced by 11.1%, 30.6% and 47.2% -PCR detection of HSV-TK gene expression in tumor tissue. There were obvious pathological changes in the experimental group compared with the control group. Conclusion The HSV-TK / GCV suicide gene system can kill cervical cancer cells in vivo, and there is obvious bystander effect.