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目的:摸索逆转录病毒介导的理想的造血细胞基因转染体系.方法:以人多药耐药基因1(mdr-1)及增强型绿色荧光蛋白基因(EGFP)为报告基因,比较了小鼠骨髓细胞与病毒包装细胞共培养、与骨髓基质细胞系TC-l共培养及单纯上清感染等不同体系的转染效率.结果:与单纯上清转染法相比,TC-1基质细胞可明显提高外源基因的转染效率.3组中,以与病毒包装细胞共培养组转染效率最高.结论:骨髓基质细胞介导基因转染的方法,既可促进靶基因的转染效率,又可避免重组辅助病毒引起的感染,具有较好的临床应用前景.
Objective: To explore retrovirus-mediated ideal hematopoietic cell transfection system.Methods: Human multidrug resistance gene 1 (mdr-1) and enhanced green fluorescent protein (EGFP) Transfection efficiency of murine bone marrow cells co-cultured with virus packaging cells, co-culture with bone marrow stromal cell line TC-1 and supernatant infection were compared.Results: Compared with the simple supernatant transfection method, TC-1 stromal cells Significantly improve the transfection efficiency of exogenous genes.In the three groups, the co-culture with virus packaging cells transfection efficiency was the highest.Conclusion: BMSCs mediated gene transfection can not only promote the transfection efficiency of target genes, But also to avoid the infection caused by recombinant helper virus, has good clinical application prospects.