Objective:To explore the effect of resistant exercise on improvement of gut microbiota regulating glucose control in mice with type 2 diabetes.Methods:Male mice with type 2 diabetes aged 8 weeks were randomly assigned to control group,aerobic training (AT)group and resistant training (RT) group.One month after the intervention,the blood glucose was measured and the feces were collected to detect gut microbiota by using 16S rRNA high-throughput sequencing method.Results:The higher proportion and abundance of Firmicutesbacteria and lower proportion and abundance of Bacteroidetesbacteria were found in AT group.The number and abundance of Akkermansia muciniphila in feces of RT group were significantly higher than those of AT and control groups.Lower level of blood glucose was observed in RT group relating to control group.Conclusion:Resistant exercise to improve gut microbiota is expected to be a new method for the prevention and treatment of type 2 diabetes.