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微藻处理猪粪沼液废水是一项污水资源化生物技术.本文以小球藻为研究对象,通过贴壁培养方式,对稀释不同倍数的猪粪沼液废水进行净化处理同时提取藻细胞油脂,旨在探究小球藻贴壁培养处理猪粪沼液废水的效果,分析小球藻耐受猪粪沼液废水的氨氮浓度.将猪粪沼液废水分别稀释1倍(原水)、2倍、5倍、10倍制成培养基.测定贴壁培养小球藻对各处理组猪粪沼液废水中COD、氨氮、总氮、总磷的去除效率及对重金属铜、锌、铁的富集效果,同时探究小球藻的油脂合成情况.结果表明,当猪粪沼液废水稀释5倍时,贴壁小球藻对COD、氨氮、总氮、总磷的净化效果最佳,其去除效率分别为:86.8%、94.1%、85.2%、84.3%;油脂含量高达32.7%;对重金属铜、锌、铁的去除效率分别为:72.9%、70.0%、73.0%;培养一个周期结束时生物产率达到4.21 g·(m~2·d)~(-1).该研究将微藻与难处理的猪粪沼液废水深度净化进行了有效的结合,为实现藻类生物燃料工艺生产及降低废水处理成本提供理论基础.
Microalgae treatment of pig manure biogas wastewater is a biological wastewater treatment technology.In this paper, Chlorella as the research object, by adherent culture, dilution of pig manure biogas slurry was purified at the same time to extract algal cell fat , Aimed at exploring the effect of chlorella culture on pig manure biogas wastewater and analyzing the ammonia nitrogen concentration of chlorella deserving pig manure biogas wastewater.The pig manure biogas wastewater was diluted 1 time (raw water), 2 times , 5 times and 10 times respectively.The removal efficiencies of COD, ammonia nitrogen, total nitrogen and total phosphorus in pig manure biogas slurry of each treatment group and the removal of heavy metal copper, zinc and iron The results showed that when the excrement of pig manure biogas slurry was diluted by 5 times, the removal efficiencies of COD, ammonia nitrogen, total nitrogen and total phosphorus were the best, The efficiencies were 86.8%, 94.1%, 85.2% and 84.3%, respectively. The lipid content was as high as 32.7%. The removal efficiencies of heavy metals copper, zinc and iron were 72.9%, 70.0% and 73.0% The yield reached 4.21 g · (m ~ 2 · d) -1. This study compared microalgae with refractory pig manure slurry Depth of water purification process was effective combination, provide a theoretical basis for the realization of algal biofuel production process and reduce the cost of wastewater treatment.