论文部分内容阅读
本文介绍用流动细胞计数(Flow cytometric)分析的方法证明KHT肉瘤经放射后可诱发细胞周期的紊乱。作者应用改进的Hochst 33342染色方法观察和测定处在G_1 S和G_2+M期的实体肿瘤细胞形成克隆的能力以及在大剂量照射原位肿瘤后杀死常氧细胞,然后测定存活的乏氧细胞的衰老特征。 Hocchst 33342是一种DNA特异性染色的方法。这种染色方法对它的活力具有最小的作用。本研究按它们DNA含量而挑选出并测定它们经放射和未经放射的实体肿瘤细胞形成克隆的能力。用体外方法测定
This article describes the use of flow cytometric analysis to demonstrate that KHT sarcoma can induce cell cycle disturbances after radiation. The authors used the modified Hochst 33342 staining method to observe and measure the ability of solid tumor cells in G 1 S and G 2 +M phases to form colonies and kill normoxic cells after high-dose irradiation of orthotopic tumors, and then to measure surviving hypoxic cells. Aging characteristics. Hocchst 33342 is a DNA-specific staining method. This staining method has the least effect on its viability. The study selected and measured their ability to form clones of irradiated and non-irradiated solid tumor cells based on their DNA content. Determined by in vitro methods