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目的:探讨泰山磐石散影响多囊卵巢综合征(PCOS)促排卵大鼠着床的可能机制。方法:取85日龄正常大鼠、克罗米芬(CC)促排卵的PCOS模型大鼠分别与雄鼠合笼,选取妊娠大鼠随机分为3组:A组(正常妊娠组)20只;B组(PCOS促排卵组)12只;C组(PCOS促排卵+泰山磐石散组)12只。妊娠d5 ELISA测定E2、P;免疫组化测定子宫内膜PR、ICAM-1水平。妊娠d8取双侧子宫计算胚泡数量。结果:胚泡着床率A组>C组>B组;胚泡着床数3组组间差异显著(P<0.01)。种植窗期E2在B组、C组高于A组,P在A组、C组高于B组,E2/P3组组间差异显著(均P<0.01);PR表达A组高于B组,ICAM-1表达A组、C组高于B组(均P<0.05);PR表达与E2、P、E2/P均无相关性(P>0.05);ICAM-1与P呈正相关,与E2/P呈负相关(P<0.01)。结论:PCOS促排卵大鼠低着床率可能与种植窗期E2、P及其比例失衡,子宫内膜PR、ICAM-1低表达有关。泰山磐石散可能通过调整种植窗期P、E2/P水平,上调子宫内膜PR、ICAM-1表达改善卵巢功能、提高子宫内膜容受性,提高早期妊娠率。
Objective: To explore the possible mechanism of Taishan Panshi Powder implicating ovulation in ovulation induced by polycystic ovary syndrome (PCOS) in rats. Methods: The rats of 85-day-old normal rats and clomiphene citrate (CC) ovulation-induced PCOS rats were respectively caged with male rats. Pregnant rats were randomly divided into three groups: group A (normal pregnancy group) Group (PCOS ovulation group) 12; Group C (PCOS Ovulation + Taishan Pan Shizai group) 12. Pregnancy d5 ELISA E2, P; immunohistochemical determination of endometrial PR, ICAM-1 levels. Pregnancy d8 bilateral uterus to calculate the number of blastocysts. Results: The rate of blastocyst implantation in group A> C> B was significantly different (P <0.01). Planting window E2 was significantly higher in group B than in group C (P <0.01); P was higher in group A than in group B (P <0.01) (P <0.05). There was no correlation between PR expression and E2, P, E2 / P (P> 0.05); ICAM-1 was positively correlated with P E2 / P was negatively correlated (P <0.01). CONCLUSION: The low implantation rate of ovulation-induced ovariectomized rats may be related to the imbalance of E2 and P in the implantation window and the low expression of PR and ICAM-1 in endometrium. Taishan Panshi Powder could improve ovarian function, improve endometrial receptivity and improve early pregnancy rate by adjusting P and E2 / P levels at the implantation window and up-regulating the expression of PR and ICAM-1 in the endometrium.