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本文采用Virella的含菌琼脂板电泳方法并加以修改,进行测定血清溶菌酶含量,用pH6.5的电泳环境,以新鲜尿为鸡蛋溶菌酶的稀释液,测定正常人与矽肺患者的血清中溶菌酶含量,所得结果与光电比浊法接近,应用本法测定的溶菌酶的最低量为2.5微克,血清用量只需10微升,操作简便,适于常规检查。
In this paper, using Virella agar plate agar plate electrophoresis method to be modified, the determination of serum lysozyme content, with pH6.5 electrophoresis environment, fresh urine as lysozyme dilution, determination of normal and silicosis in patients with lysozyme Enzyme content, the results obtained and the photoelectric turbidimetric approach, the minimum amount of lysozyme determined by this method is 2.5 micrograms, the amount of serum only 10 microliters, easy to operate, suitable for routine examination.