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Objective:To investigate the effects of follistatin(rhFS-288) on biosynthesis andsecretion of testosterone in rat Leydig cell in vitro.Methods:Leydig cells were isolated from Wistar rat testes by a discontinuous Per-coll gradient procedure.Purified cells were incubated in 24-well plate(10~5 cell/ml/well)and maintained for 24 h in a CO_2 incubator,rhFS-288 and Ca~(2+) were added to the wellsindependently or jointly in both baseline (without hCG) and stimulation condition (1.0IU/ml of hCG) to observe the change of testosterone concentration in the media.Results:rhFS-288 showed a dose-dependent inhibiting effect on testosterone releasein baseline and stimulating condition.Ca~(2+) presented inhibitory effect either.Whereas,escape phenomenon emerged while Ca~(2+) concentration reached to 100 mmol/L.A com-bination of rhFS-288 with Ca~(2+) displayed a dose-dependent inhibition on testosterone se-cretion.Conclusion:rhFS-288 inhibits testosterone secretion in a dose-dependent manner.Calcium is thought to be the second messenger of FS action.The mechanism of escapephenomenon during high dose of Ca~(2+) along is unknown.
Objective: To investigate the effects of follistatin (rhFS-288) on biosynthesis and secretion of testosterone in rat Leydig cell in vitro. Methods: Leydig cells were isolated from Wistar rat testes by a discontinuous Per-coll gradient procedure. Purified cells were incubated in 24 -well plate (10-5 cells / ml / well) and maintained for 24 h in a CO2 incubator, rhFS-288 and Ca2 + were added to the wells in dependent or jointly in both baseline (without hCG) and stimulation condition (1.0 IU / ml of hCG) to observe the change of testosterone concentration in the media. Results: rhFS-288 showed a dose-dependent inhibiting effect on testosterone releasein baseline and stimulating condition. Ca 2+ presented inhibitory effect either. Whereas, while the Ca 2+ concentration reached 100 mmol / LA com-bination of rhFS-288 with Ca 2+ (a-dependent inhibition on testosterone se-cretion. Conlusion: rhFS-288 inhibits testosterone secretion in a dose-dependent manner. Calcium is thought to be the second messenger of FS action.The mechanism of escapephenomenon during high dose of Ca ~ (2+) along is unknown.