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目的探讨心肌缺血预处理(IP)对心肌中肌钙蛋白T(cTnT)表达的影响。方法将45只兔随机分为3组,即假手术组(只开胸、穿线不结扎冠脉)、直接梗死组(开胸、并结扎冠脉)及IP组(结扎冠脉5 min,再灌注10 min,反复3次)。于术后15 min、30 min、1 h、3 h及5 h处死动物,切取心肌,用免疫组化染色法检测心肌中cTnT的表达,并进行计算机图像分析。在高倍光镜下,测量心肌中不表达cTnT(cTnT-)的面积,取其平均值进行统计学分析。结果比较同一时段3个组cTnT-的面积,发现IP组cTnT-的面积大于假手术组,但小于直接梗死组(P<0.05)。结论IP明显减少缺血心肌的cTnT面积。
Objective To investigate the effect of myocardial ischemic preconditioning (IP) on the expression of cardiac troponin T (cTnT) in myocardium. Methods 45 rabbits were randomly divided into 3 groups: sham operation group (thoracotomy only, threading without ligating coronary artery), direct infarction group (thoracotomy and ligating coronary artery) and IP group (ligating coronary artery for 5 min Perfusion 10 min, repeated 3 times). The animals were sacrificed at 15 min, 30 min, 1 h, 3 h and 5 h after operation, and the myocardium was excised. The expression of cTnT in myocardium was detected by immunohistochemical staining and analyzed by computer image. Under high-power light microscope, the area of cTnT (cTnT-) that is not expressed in myocardium was measured and the average value was taken for statistical analysis. Results Compared with the cTnT-3 area in the same time period, the area of cTnT-1 in IP group was larger than that in sham-operated group but less in direct infarct group (P <0.05). Conclusions IP significantly reduces cTnT area of ischemic myocardium.