[目的]探讨p21蛋白与胃癌术后患者FLO方案辅助化疗疗效之间的关系。[方法]选取966例胃癌组织标本,采用组织芯片技术检测胃癌组织中细胞核内与胞浆中p21蛋白表达,应用Kaplan-Meier方法绘制生存曲线,Log-rank检验比较生存率。[结果]p21在核中普遍低表达,而在浆中却呈高表达。浆p21高表达、核p21低表达者术后采取FLO方案化疗均能从总生存期(OS)中获益(P<0.05);相反,浆p21低表达、核p21高表达者在OS上未能获益(P>0.05)。[结论 ]p2l蛋白表达可以预测胃癌术后患者对FLO方案化疗疗效,可为胃癌术后患者的个体化治疗提供临床理论依据。 [Objective] To investigate the relationship between p21 protein and the efficacy of adjuvant chemotherapy with FLO regimen in postoperative patients with gastric cancer. [Method] A total of 966 specimens of gastric cancer tissues were selected. The expression of p21 protein in nucleus and cytoplasm of gastric cancer tissues was detected by tissue microarray. The survival curves were drawn by Kaplan-Meier method. The survival rate was compared by Log-rank test. [Result] p21 was generally low in the nucleus and high in the pulp. Pulmonary p21 was overexpressed, whereas p21 low nuclear p21 expression was associated with an overall survival (OS) after surgery with FLO chemotherapy (P <0.05). On the contrary, low expression of p21 and high expression of nuclear p21 did not occur on OS Can benefit (P> 0.05). [Conclusion] The expression of p21 protein can predict the effect of FLO regimen in postoperative patients with gastric cancer, and provide a theoretical basis for the individualized treatment of postoperative patients with gastric cancer.