目的 :制备凝血酶调节蛋白 (thrombomodulin ,TM)抗体。方法 :原核表达目的蛋白 ,将表达的融合蛋白纯化后免疫小鼠。以EVC30 4包板的cell ELISA检测抗体滴度 ,用Westernblot和免疫组化鉴定抗体特异性。结果 :目的蛋白在大肠杆菌中大量表达形成包涵体 ,将表达蛋白纯化并复性后免疫小鼠 ,3次免疫后得抗体效价为 1∶4 0 0 0 ,经加强免疫后抗体效价达到1∶80 0 0 ;特异性较好。结论 :在天然TM提取纯化困难的情况下 ,通过原核表达目的蛋白制备抗体是可行的 Objective: To prepare thrombomodulin (TM) antibody. Methods: Prokaryotic expression of the target protein, the fusion protein was purified after immunization of mice. Antibody titers were detected by cell ELISA with 4-kit EVC30 4-kit and antibody specificity by Western blot and immunohistochemistry. Results: The target protein was highly expressed in E. coli to form inclusion bodies. The expressed protein was purified and renatured and then the mice were immunized. After 3 immunizations, the antibody titer was 1: 400 and the antibody titer reached 1:80 0 0; better specificity. Conclusion: It is feasible to prepare antibodies by prokaryotic expression of the target protein in the extraction and purification of natural TM