Ctr1mRNA表达水平与细胞内CuZn-SOD活性的相关性

来源 :黑龙江八一农垦大学学报 | 被引量 : 0次 | 上传用户：chenpenghust

【摘要】

：

采用半定量RT-PCR法检测不同铜水平对猪传代肾细胞(PK15)中特异性铜转运蛋白Ctr1基因mRNA表达水平的影响,以β-actin为内参考,对各试验组cDNA同时进行PCR扩增;采用黄嘌呤氧化

【作者】

：

付世新夏成李鹏沈冰蕾王长远

【机构】

：

黑龙江八一农垦大学动物科技学院,黑龙江八一农垦大学食品学院

【出处】

：

黑龙江八一农垦大学学报

【发表日期】

：

2004年期

【关键词】

：

铜 PK15细胞 Ctr1mRNA CuZn-SOD活性

【基金项目】

：

黑龙江省教育厅课题;

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采用半定量RT-PCR法检测不同铜水平对猪传代肾细胞(PK15)中特异性铜转运蛋白Ctr1基因mRNA表达水平的影响,以β-actin为内参考,对各试验组cDNA同时进行PCR扩增;采用黄嘌呤氧化酶法对各实验组细胞内CuZn-SOD活性进行检测.猪肾PK15细胞Ctr1基因mRNA表达量在铜添加量在7.8～62.5 μmoL·L-1范围时,其表达量呈双相反应;实验各组细胞内CuZn-SOD活性均高于对照组(P

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Ctr1mRNA表达水平与细胞内CuZn-SOD活性的相关性

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