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分析在-1℃温度中处理1、2和3d的转昆虫抗冻蛋白基因MpAFP149烟草和野生型烟草亚细胞显微结构变化的结果表明,转基因烟草和野生型烟草的超微结构有差异,尤其是细胞膜、叶绿体和线粒体的膜。转基因烟草细胞器的膜结构伤害程度明显低于野生型烟草,这提示低温条件下转基因烟草中的抗冻蛋白可能有保护细胞膜和细胞器膜结构的作用,由此降低了低温的伤害。
The results of analyzing the changes of the sub-cellular microstructure of MpAFP149 tobacco and wild-type tobacco that treated 1, 2 and 3d transgenic tobacco at -1 ℃ showed that the ultrastructures of transgenic tobacco and wild-type tobacco were different, especially Is a membrane of membranes, chloroplasts and mitochondria. The injury of membrane structure of transgenic tobacco organelles was significantly lower than that of wild type tobacco, suggesting that antifreeze proteins in transgenic tobacco may have the function of protecting the cell membrane and organelle membrane structure under low temperature, thus reducing the damage of low temperature.