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目的分析Toll样受体(TLR)家族在人肝细胞癌(HCC)中的表达模式,探讨TLR参与肝细胞癌发生发展的可能机制及临床价值。方法从癌症基因组图谱(TCGA)数据库下载HCC患者的临床数据和基因表达数据,采用生物信息学方法分析TLR家族表达与HCC临床病理学参数的相关性及对预后的影响。结果与正常组织相比,人HCC组织中除TLR5、TLR7和TLR9以外,其他TLR的表达量均下调;TLR的表达量高低对HCC患者的TNM分期及预后无显著影响。通过TLR与炎性体(inflammasome)相关因子的相关性分析,发现所有的TLR都与胱天蛋白酶1(caspase-1)和白细胞介素1β(IL-1β)呈明显正相关,TLR3与IL-18未显示出明显的相关性,TLR3、TLR4、TLR6与含CARD结构域凋亡相关斑点样蛋白(ASC)也无明显的相关性。结论 HCC组织部分TLR表达下调,与炎性体相关因子正相关。
Objective To analyze the expression pattern of Toll-like receptor (TLR) family in human hepatocellular carcinoma (HCC) and to explore the possible mechanism and clinical value of TLR in the development of hepatocellular carcinoma. Methods The clinical data and gene expression data of HCC patients were downloaded from Cancer Genome Atlas (TCGA) database. The correlation between TLR family members and HCC clinical pathological parameters and prognosis were analyzed by bioinformatics methods. Results Compared with normal tissues, the expression of other TLRs in human HCC tissues was down-regulated except TLR5, TLR7 and TLR9. The expression of TLR had no significant effect on the TNM stage and prognosis of HCC patients. Correlation analysis between TLR and inflammasome showed that all the TLRs were positively correlated with caspase-1 and IL-1β, while TLR3 and IL- 18 did not show any obvious correlation. TLR3, TLR4 and TLR6 also showed no significant correlation with CARD-associated apoptosis-associated speckle-like protein (ASC). Conclusion The expression of TLR in HCC tissues is down-regulated, which is positively correlated with inflammatory factors.