取4～20月令的Wistar系雄鼠,取出附睾,冼净、从尾部切开一小口,将一滴精液置于37℃的5毫升大鼠受精培养剂(RFM)中备用.密度为20——80万/毫升.射出的精液是用吸管从交配过的雌鼠子宫中取出,置于RFM中.精子密度与附睾中取出的一样.过滤方法:采取McGrath等1977年的柱状有孔玻璃珠法.卵细胞是从排卵过速的未成年雌性大鼠的输卵管壶中取出.这些雌鼠在70和14小时前,预先用20国际单位孕马血清和25国际单位人类绒毛膜促性腺激素皮下注射.与精液相混后的第二天,在相差显微镜下检查卵细胞是否受精.结果发现:附睾精子与射出精子的受精率有差别,用5只雄鼠的附睾精子使50%(404/810)卵细胞受精.经外科手术移去第一只附睾后,从同一只雄鼠的射出精液使21%(118/571)卵细胞受精,二者 Wistar males from April to May were taken, epididymides were removed, and a small cut was made from the tail to place one drop of semen in 5 ml of rat fertilization medium (RFM) at 37 ° C. The density was 20- -80 million / ml.The ejaculated sperm is removed from the female uteri of the mating female mice by using a pipette, placed in the RFM, and the density of the spermatozoa is the same as that removed from the epididymis. Filtration method: A cylindrical filter with cylindrical glass beads of McGrath et al. Method was used to remove oocytes from the oviduct pot of ovulation-prone, juvenile female rats that had been pre-injected with 20 IU of pregnant mare serum and 25 IU of human chorionic gonadotropin subcutaneously 70 and 14 hours prior On the second day after mixing with semen, the egg was examined under a phase contrast microscope to find out whether the fertilized egg was differentiated from the spermatozoa. The epididymal spermatozoa of five male mice were used to fertilize 50% (404/810) eggs After surgical removal of the first epididymis, 21% (118/571) of the oocytes were fertilized by injection of semen from the same male, both