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1989年9月份,安阳县发生了肝炎流行,我们用微量 ELI SA 双抗体夹心法检测 HAV—IgM,取得较满意的结果,现报告如下:材料和方法:1、材料:羊抗人 U 链抗体,甲肝抗原HAAg,酶标记 HA V—IgG,抗 HA V—IgM 阳、阴性血清.2.方法:抗 U 链抗体用 P H9.6 0.05M碳酸盐缓冲液稀释后加到聚苯乙烯板中,每孔100微升4℃过夜,用 PBS—Tween20洗一次甩千;加1%牛血清白蛋白 PBS—Tween20,每孔100微升37℃温育2.5小时,P
September 1989, Anyang County, hepatitis epidemic, we use a small amount of ELI SA double antibody sandwich assay of HAV-IgM, and achieved more satisfactory results are as follows: Materials and Methods: 1, material: sheep anti-human U chain antibody , HA antigen of HA, enzyme-labeled HA V-IgG, anti-HA V-IgM positive and negative serum.2.Methods: The anti-U chain antibody was diluted with P H9.6 0.05M carbonate buffer and added to the polystyrene plate In each well 100 μl 4 ℃ overnight, washed once with PBS-Tween20 Thousand rejection; plus 1% bovine serum albumin PBS-Tween20, 100 μl per well incubated at 37 ℃ for 2.5 hours, P