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目的:建立高效液相色谱法测定人血浆中阿莫地喹的浓度.方法:分析柱采用Kromasil C1g(150 mm×4.6 mm,5 μm),流动相为甲醇:水:三乙胺:磷酸:(21:77.5:1:0.5),流速为1.0 mL·min-1,检测波长为294 nm,内标为羟氯喹.结果:阿莫地喹和羟氯喹的保留时间分别为5.82 min,8.56 min.该法在10～1 000μg·L-1浓度范围内有良好的线性关系(r=0.999 8,n=9),最低检测限为5μg·L-1,提取回收率为75.5%～82.7%,方法回收率为97.0%～104.8%.日内精密度的RSD＜6.0%,日间精密度的RSD＜7.5%.结论:该法简单,灵敏适合于阿莫地喹的药动学研究.“,”AIM:To establish HPLC method for the determination of amodiaquine in human plasma.METHODS: Amodiaquine and internal standard(hydroxychloroquine) were analyzed on C18 column(150 mm ×4.6 mm, 5 μm) with methanol: water:triethylamine: orthophosphoric acid (21:77.5:1:0.5 )as mobile phase at the flow rate of 1.0 mL · min-1The UV detector was set at 294 nm. RESULTS: The retention times of amodiaquine and internal standard were 5.82, 8.56 min, respectively. The calibration curve was linear in the range from 10 to 1 000 μg ·L- 1 ( r = 0.999 8, n = 9 ). The limit of quantitation was5 μg· L-1. The extraction recovery was between 75.5 % and 82.7 %, and the methodological recovery was between 97.0 % and 104.8 %. The intra-day and inter-day RSD were less than 6.0 % and 7.5 %, respectively. CONCLUSION: This HPLC method is simple, sensitive and suitable for pharmacokinetic study of amodiaquine.