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目的构建携带有多聚免疫球蛋白受体(pIgR)的转基因鼠,使其能以近乎自然的状态感染EBV,观察EBV在鼻咽部病变过程中的作用。方法采用角质上皮特异性启动子ED-L2调控的pIgR基因,用显微注射方法将其导入受精卵中,使该基因能在F0代转基因鼠鼻咽部特异性表达。结果PCR检测F0代pIgR基因阳性率达37.5%(6/16),Southern杂交F0代pIgR基因阳性率为12.5%(2/16)。结论表达多聚免疫球蛋白受体的转基因动物有望成为研究EBV病毒致病机制的一种新的动物模型。
Objective To construct a transgenic mouse carrying polyIgR (pIgR) and make it capable of infecting EBV in a nearly natural state and to observe the role of EBV in the pathogenesis of nasopharyngeal lesions. Methods The pIgR gene regulated by ED-L2 of keratinocyte-specific promoter was introduced into zygotes by microinjection to make the gene express specifically in the nasopharynx of F0 generation transgenic mice. Results The positive rate of pIgR gene in F0 generation was 37.5% (6/16) by PCR. The positive rate of pIgR gene in F0 generation by Southern hybridization was 12.5% (2/16). Conclusions Transgenic animals expressing poly-immunoglobulin receptors are expected to become a new animal model for studying the pathogenesis of EBV virus.