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目的:观察不同时期肝硬化大鼠下腔静脉与门静脉血中一氧化氮(NO)浓度变化,探索其在门脉高压症发生发展过程中的作用。方法:制备四氯化碳诱导的肝硬化大鼠模型,在第15天、第30天、第52天取大鼠下腔静脉及门静脉血检测NO浓度。结果:正常及肝硬化Ⅰ期、Ⅱ期、Ⅲ期大鼠下腔静脉和门静脉血中NO浓度分别为71.088±8.739、102.821±6.783、122.499±4.605、155.843±10.328μmol/L和87.025±7.858、115.377±7.935、146.222±8.360、182.811±8.449μmol/L。门静脉血和下腔静脉血中NO浓度随着肝脏病变的加重而逐渐增高,不同时期之间各组大鼠门静脉和下腔静脉血NO浓度的增高有显著性。而且,各组大鼠门静脉血中NO浓度均显著高于同组大鼠的下腔静脉血中NO的浓度,结论:实验性肝硬化大鼠门静脉血和下腔静脉血中CO在肝硬化门脉高压高动力循环发生发展中起着重要作用,尤其以门静脉血中NO为更重要。
Objective: To observe the changes of nitric oxide (NO) in the blood of inferior vena cava and portal vein of rats with cirrhosis at different stages and to explore its role in the occurrence and development of portal hypertension. Methods: CCl4-induced cirrhotic rat model was prepared. NO concentration was measured in the inferior vena cava and portal vein on the 15th, the 30th and the 52nd day. Results: NO concentrations in inferior vena cava and portal vein of normal and cirrhosis stage Ⅰ, Ⅱ, and Ⅲ were 71.088 ± 8.739,102.821 ± 6.783,122.499 ± 4.605,155.843 ± 10.328μmol / L and 87.025 ± 7.858, respectively, 115.377 ± 7.935, 146.222 ± 8.360, 182.811 ± 8.449 μmol / L. The concentration of NO in portal vein blood and in inferior vena cava blood increased gradually with the increase of liver disease. The increase of NO in portal vein and in inferior vena cava of rats in different periods was significant. Moreover, the concentration of NO in portal vein blood of rats in each group was significantly higher than that of NO in the blood of inferior vena cava of rats in the same group. Conclusion: The CO in portal vein blood and in inferior vena cava of experimental cirrhotic rats was significantly higher than that in cirrhosis Hypertonic hyperdynamic circulation plays an important role in the development, especially the portal vein blood NO is more important.