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【目的】预测和鉴定梅毒螺旋体(Tp)Tp92蛋白的B细胞表位,为深入探讨这些表位在梅毒表位疫苗中的作用奠定基础。【方法】采用Mobyle、ABCpred和IEDB在线软件综合分析预测Tp92蛋白的B细胞表位,人工合成6条表位多肽,以梅毒患者/感染兔血清(同时设健康人/兔血清对照)为标本,用间接ELISA法鉴定预测Tp92蛋白B细胞表位的免疫反应性。【结果】软件预测显示,Tp92蛋白的P1(24-39AA)、P2(332-347AA)、P3(520-536AA)、P4(575-588AA)、P5(103-118AA)、P6(694-712AA)氨基酸序列可能为其B细胞表位。间接ELISA分析表明,预测的P1、P3、P5和P6均与梅毒患者/感染兔血清呈阳性反应,而与健康人/兔血清不反应。【结论】本研究初步得出以下结论:P1、P3、P5和P6均为Tp92蛋白潜在的特异性B细胞表位,尤其是P3和P6免疫反应性最强。
【Objective】 To predict and identify the B cell epitopes of Tp92 protein of Treponema pallidum (Tp), and lay a foundation for further study on the roles of these epitopes in the syphilis epitope vaccine. 【Methods】 B-cell epitopes of Tp92 protein were predicted and synthesized by on-line software of Mobyle, ABCpred and IEDB. Six epitope peptides were synthesized and the syphilis patients / infected rabbit sera (with healthy human / rabbit serum control) Indirect ELISA was used to identify the immunoreactivity of Tp92 protein B cell epitopes. 【Results】 The software prediction showed that P1 (24-39AA), P2 (332-347AA), P3 (520-536AA), P4 (575-588AA), P5 (103-118AA), P6 (694-712AA ) The amino acid sequence may be its B-cell epitope. Indirect ELISA analysis showed that the predicted P1, P3, P5, and P6 were both positive for syphilis patients / infected rabbit sera but not for healthy human / rabbit sera. 【Conclusion】 The preliminary results of this study are as follows: P1, P3, P5 and P6 are potential specific B cell epitopes of Tp92 protein, especially P3 and P6 are the most immunoreactive.