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目的 检测抑癌基因p5 3 ,血管内皮生长因子 (VEGF)和微血管密度 (MVD)在乳腺癌及乳腺癌旁组织中的表达 ,探讨其相关性及其在乳腺癌发生发展过程中的作用。方法 采用免疫组织化学SP法检测 110例乳腺癌及其癌旁乳腺组织中p5 3 ,VEGF表达水平 ,比较MVD的大小。结果 VEGF ,p5 3在乳腺癌组织中的阳性表达率分别为 90 0 % ( 99/ 110 ) ,42 7% ( 4 7/ 110 ) ;癌旁乳腺组织中阳性表达率则分别为 3 5 5 % ( 3 9/ 110 ) ,4 5 % ( 5 / 110 ) ,两者间具有高度显著性差异 (P <0 0 1)。乳腺癌组织微血管密度MVD均值为 ( 16 4± 12 3 ) ,癌旁乳腺组织则为 ( 7 3± 6 8) ,两者间具有高度显著性差异 (P <0 0 1)。MVD值在p5 3、VEGF阳性表达的组织中明显高于p5 3、VEGF阴性表达的组织 ,二者比较差异显著 (P <0 0 1、P <0 0 5 )。结论 联合检测p5 3、VEGF和MVD的表达可作为判断乳腺癌生物学行为和预后的重要指标。
Objective To detect the expression of tumor suppressor gene p53, vascular endothelial growth factor (VEGF) and microvessel density (MVD) in breast cancer and paracancerous tissues, and to explore the correlation and its role in the development and progression of breast cancer. Methods Immunohistochemical SP method was used to detect the expression of p5 3 and VEGF in 110 cases of breast cancer and adjacent normal breast tissues. The size of MVD was compared. Results The positive rates of VEGF and p5 3 in breast cancer tissues were 90 0% (99/110) and 42 7% (47/110), respectively. The positive rates of VEGF and p5 3 in breast cancer tissues were 35.5% (39/110) and 45% (5/110), respectively, with a highly significant difference (P <0.01). The MVD of breast cancer tissue was (16 4 ± 12 3) and that of paracancer breast tissue was (73 ± 68) respectively, with a highly significant difference (P <0.01). MVD was significantly higher in p5 3 and VEGF positive tissues than in p5 3 and VEGF negative tissues (P <0.01 or P <0.05). Conclusion The combined detection of p5 3, VEGF and MVD expression may be used as an important index to judge the biological behavior and prognosis of breast cancer.