论文部分内容阅读
目的:研究GRTH基因c.852C/T位点的多态性与无精症的关系.方法:应用PCR-RFLP技术,在228例无精症患者和259例正常生育男性中,对GRTH基因c.852C/T单核苷酸多态位点的多态分布与无精症的相关性进行研究.结果:无精症患者和正常男性间c.852C/T的等位基因T的频率存在统计学差异(32.2%vs26.2%,P=0.04);在无精症患者中的基因型TT的频率显著高于正常男性(9.6%vs4.6%,P=0.03).结论:GRTH基因c.852C/T的多态分布与无精症相关,基因型TT增加无精症的易感性,可能是人类生精阻碍的一个风险因子.
Objective: To investigate the relationship between polymorphism of c.852C / T polymorphism of GRTH gene and azoospermia.Methods: PCR-RFLP was used to detect the expression of GRTH gene c in 228 azoospermic men and 259 normal fertile men The relationship between polymorphism of .852C / T SNP and azoospermia was studied.Results: The allele T frequency of c.852C / T between azoospermia and normal male was statistically significant (32.2% vs26.2%, P = 0.04). The frequency of genotype TT in patients with azoospermia was significantly higher than that in normal males (9.6% vs.4.6%, P = 0.03) The polymorphism of .852 C / T is associated with azoospermia, and genotype TT increases the susceptibility to azoospermia and may be a risk factor for the inhibition of sperm production in humans.