论文部分内容阅读
目的探讨移植肾组织中转化生长因子β1(TGF-β1)和基质金属蛋白酶2(MMP-2)的表达及IV型胶原沉积与慢性移植肾肾病(CAN)的关系。方法对18例CAN患者进行了移植肾切除术。光镜下观察切除的肾组织标本并根据其纤维化的程度进行分期,用免疫组织化学技术和图像分析法检测移植肾组织中TGF-β1和MMP-2表达量及IV型胶原沉积情况,并分析患者血肌酐(Cr)和尿素氮(BUN)的水平与移植肾纤维化程度的关系。结果随着患者移植肾纤维化程度的加重,血清肌酐、尿素氮及肾组织中TGF-β1、IV型胶原基因表达量均增加,MMP-2表达量于移植肾纤维化早期明显升高,并随移植肾纤维化程度加重而逐渐降低。结论移植肾组织中TGF-β1表达量增高及IV型胶原沉积可以促进CAN的进程,而MMP-2则可抑制其进展。
Objective To investigate the expression of transforming growth factor β1 (TGF-β1) and matrix metalloproteinase-2 (MMP-2) in renal allograft and the relationship between type IV collagen deposition and chronic allograft nephropathy (CAN) Methods 18 cases of CAN patients underwent nephrectomy. The specimens of renal allograft were observed under light microscope and staged according to the degree of fibrosis. The expression of TGF-β1 and MMP-2 and the deposition of type IV collagen in renal allograft tissues were detected by immunohistochemistry and image analysis The relationship between serum creatinine (Cr) and blood urea nitrogen (BUN) and the degree of renal allograft fibrosis was analyzed. Results As the degree of renal interstitial fibrosis increased, the expression of TGF-β1 and type IV collagen in serum creatinine, urea nitrogen and renal tissue increased, and the expression of MMP-2 increased significantly in early stage of renal allograft fibrosis With the severity of graft kidney fibrosis and gradually decreased. Conclusion The increased expression of TGF-β1 and type IV collagen deposition in renal allograft can promote the progression of CAN, while MMP-2 inhibits the progression of CAN.