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以不同基因型材料的西瓜品种为试材，用ＭＳ、Ｍｉｌｌｅｒ、Ｎ６与不同激素配比的培养基，初步建立了西瓜离体组培再生体系。结果表明，不同浓度的ＭＳ培养基均可长出无菌苗，但随着ＭＳ培养基营养元素浓度的增大，无菌苗生长发育受到延迟和抑制作用加强，其中以 １／２ ＭＳ＋ ０．５～２ ｍｇ／Ｌ ＢＡ＋ ０． ｍｇ／Ｌ ＮＡＡ长出的无菌苗作外植体诱导愈伤组织最快，改良的 ＭＳ（ＭＳＩ＋ ２ ｍｇ／Ｌ ＢＡ＋０．１ｍｇ／ＬＮＡＡ）是西瓜愈伤组织诱导的最适培养基，改良的ＭＳ（ＭＳＩ＋２ｍｇ／ＬＢＡ＋０．１ｍｇ／ＬＮＡＡ＋５ ｍｇ／ＬＡｇＮＯ３）是外植体诱导分化不定芽的最适培养基，离子束等物理因子处理愈伤组织发现对诱导分化不定芽具有刺激和促进作用。
Watermelon cultivars with different genotypes were used as materials to establish a regeneration system of in vitro tissue culture of watermelon with medium supplemented with MS, Miller, N6 and different hormones. The results showed that aseptic seedlings could be grown in different concentrations of MS medium, but with the increase of nutrient concentration in MS medium, the growth and development of aseptic seedlings were delayed and inhibited, with 1/2 MS +0. 5 ~ 2 mg / L BA + 0. The explants induced by aseptic shoots with mg / L NAA had the fastest callus induction. The modified MS (MSI + 2 mg / L BA + 0.1 mg / L NAA) was the optimum medium for callus induction of watermelon. MS (MSI + 2mg / LBA + 0.1mg / L NAA + 5mg / LAgNO3) was the most suitable medium for explant to induce adventitious buds. The treatment of callus with physical factors such as ion beam could stimulate and promote the differentiation of adventitious buds.