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目的:建立一种新的快速诊断肾移植受者人巨细胞病毒(HCMV)活动性感染的方法。方法:运用免疫组织化学的催化信号扩增法通过抗巨细胞病毒pp65单克隆抗体AAC10对外周血白细胞中的人巨细胞病毒pp65抗原进行标记识别。结果:检测61例肾移植受者中,HCMVpp65抗原阳性36例,活动性感染率为59%(36/61),有症状的HCMV20例,占所有受者33%(20/61),平均抗原指数(98±31)个/2×105WBC;而无症状的活动性感染HCMVpp65抗原指数平均为(21±22)个/2×105WBC。同时检测50名健康人,结果pp65抗原全为阴性。结论:该法敏感、简便,可作为肾移植后HCMV病的早期诊断并可指导抗病毒治疗。
Objective: To establish a new method for rapid diagnosis of human cytomegalovirus (HCMV) active infection in renal transplant recipients. Methods: The anti-cytomegalovirus pp65 monoclonal antibody AAC10 was used to identify the human cytomegalovirus pp65 antigen in peripheral blood leukocytes by using the immunohistochemical catalytic signal amplification method. Results: Of the 61 renal transplant recipients, 36 were positive for HCMV pp65 antigen, the rate of active infection was 59% (36/61), symptomatic HCMV was 20 (33%, 20/61), and the average antigen (98 ± 31) / 2 × 105WBC respectively, while the asymptomatic HCMV pp65 antigen index was (21 ± 22) / 2 × 105WBC. At the same time testing 50 healthy people, the results pp65 antigen all negative. Conclusion: The method is sensitive and simple, which can be used as an early diagnosis of HCMV after renal transplantation and can guide antiviral therapy.