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目的:microRNAs(miRNAs)的异常表达与多种疾病密切相关,并有可能用于肿瘤治疗。本研究探讨了miR-143在人膀胱癌细胞中的作用及机制,为膀胱癌的临床诊治提供参考。方法:采用体外培养的T24细胞株为研究对象,按照处理方式分为空白对照组(T24)、阴性对照组(NC)、miRNA-143转染组(miR-143)以及si-COX-2转染组(si-COX-2)。3H-thymidine法和Transwell趋化实验检测T24细胞增殖和迁移能力,免疫印迹法检测COX-2蛋白表达变化。结果:miR-143和si-COX-2转染T24细胞48h-72h后,细胞增值能力较正常T24细胞相比下降36%-49%(P<0.01),迁移能力下降81%。免疫印迹结果表明,si-COX-2或miR-143转染的T24细胞内源性COX-2表达水平显著减少至正常T24细胞表达水平的0.39和0.31倍(P<0.01)。结论:miR-143可降低膀胱癌T24细胞增值力和侵袭力,并抑制COX-2表达。miR-143可能通过COX-2通路发挥对膀胱癌T24细胞的增殖和侵袭的抑制作用。研究结果更加明确了microRNA在癌症中的功能,提示miR-143可作为膀胱癌的治疗候选药物。本研究为探索肿瘤生物标志物和治疗提供新的启示。
Aims: The abnormal expression of microRNAs (miRNAs) is closely related to various diseases and may be used in cancer therapy. This study explored the role and mechanism of miR-143 in human bladder cancer cells and provided a reference for the clinical diagnosis and treatment of bladder cancer. Methods: T24 cells cultured in vitro were divided into blank control group (T24), negative control group (NC), miRNA-143 transfected group (miR-143) and si-COX- Dye group (si-COX-2). The proliferation and migration of T24 cells were detected by 3H-thymidine assay and Transwell chemotaxis assay. The expression of COX-2 protein was detected by Western blotting. Results: After 48h-72h transfection of miR-143 and si-COX-2 cells, the cell proliferation ability decreased by 36% -49% (P <0.01) and the migration ability decreased by 81% compared with normal T24 cells. Western blot results showed that the expression of endogenous COX-2 in T24 cells transfected with si-COX-2 or miR-143 was significantly reduced to 0.39 and 0.31 times that of normal T24 cells (P <0.01). Conclusion: miR-143 can reduce the proliferation and invasiveness of bladder cancer T24 cells and inhibit the expression of COX-2. miR-143 may exert an inhibitory effect on the proliferation and invasion of bladder cancer T24 cells through the COX-2 pathway. The results more clearly the function of microRNA in cancer, suggesting that miR-143 can be used as a candidate drug for bladder cancer. This study provides new insights into exploring tumor biomarkers and therapeutics.