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目的:观察熊果酸和熊果酸联合枸杞对小鼠肝癌前病变的防护作用。方法:将40只小鼠分为4组:正常组、模型组、熊果酸组和联合用药组,每组10只。除正常组外其余3组小鼠均每周腹腔注射1次二乙基亚硝胺复制小鼠肝癌前病变模型,同时正常和模型组小鼠每天灌胃生理盐水,熊果酸组小鼠每天灌胃熊果酸,联合用药组小鼠每天灌胃熊果酸和枸杞子,均连续10周。腹主动脉取血检测血清肝功能指标,固定光镜观察肝组织学变化。结果:与对照组比较,模型组小鼠肝脏同时存在变质与修复性病变,增生肝细胞具有异型性,血清ALT、AST、ALP明显升高(P<0.01),GST升高(P<0.05)。熊果酸组小鼠肝损伤较轻,增生肝细胞轻度异型性,ALT、AST、GST较模型组显著降低(P<0.01),ALP降低(P<0.05);联合用药组小鼠ALT、AST较模型组显著降低(P<0.01),GST降低(P<0.05),ALP有降低的趋势(P>0.05)。结论:熊果酸对诱导性小鼠肝癌前病变具有防治作用;熊果酸与枸杞联合应用疗效更好。
Objective: To observe the protective effect of ursolic acid and ursolic acid combined with medlar on precancerous lesions of liver cancer in mice. Methods: Forty mice were divided into 4 groups: normal group, model group, ursolic acid group and combination group, 10 in each group. Except the normal group, the remaining three groups of mice were intraperitoneally injected with diethylnitrosamine once a week for mice model of precancerous lesions of hepatocellular carcinoma. Meanwhile, the normal and model groups of mice were orally gavaged with normal saline. The ursolic acid-treated mice Ursolic acid stomach, combined with mice were administered intraperitoneal ursolic acid and medlar every day for 10 weeks. Abdominal aorta blood to detect serum liver function indicators, fixed light microscopy liver histological changes. Results: Compared with the control group, the liver of the model group had both metamorphic and reparative lesions. The hepatocytes were characterized by atypia. The levels of ALT, AST and ALP were significantly increased (P <0.01) and the GST was increased (P <0.05) . Compared with the model group, the ALA, AST and GST decreased significantly (P <0.01) and the ALP decreased (P <0.05) in the ursolic acid group, while ALT, AST, AST decreased significantly (P <0.01), GST decreased (P <0.05) and ALP decreased (P> 0.05). Conclusion: Ursolic acid has a preventive and therapeutic effect on the precancerous lesions induced by hepatocarcinoma in mice. Ursolic acid and wolfberry have better curative effect.