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利用还原法制备Ag纳米粒子,利用Stber法成功制备了不同壳层厚度的核壳结构Ag@SiO_2纳米粒子。并利用重力沉积方法在玻璃基片上构筑了纳米粒子二维自组装膜结构,探讨了该自组装体对荧光物质的等离子体共振增强荧光性能。结果表明:通过简单的包覆方法,可控制得到不同壳层厚度的Ag@SiO_2纳米粒子;Ag@SiO_2纳米粒子的二维组装薄膜对荧光物质增强作用具有显著的距离依赖性,当壳层厚度为(5±1)nm时,纳米粒子薄膜对罗丹明B荧光增强作用达到最大,增强倍数约为5倍;当壳层厚度大于22 nm时,纳米粒子薄膜对罗丹明B几乎没有荧光增强效应。以Cy5~羊抗兔蛋白为荧光物质,进一步证实了该核壳结构Ag@SiO_2组装体的有效增强荧光性能。
Ag nanoparticles were prepared by reduction method and Ag @ SiO 2 nanoparticles with different shell thickness were successfully prepared by Stöber method. The two-dimensional self-assembled monolayer of nanoparticles was constructed on the glass substrate by gravimetric deposition method. The plasmon resonance enhanced fluorescence of the self-assembly was also discussed. The results show that the Ag @ SiO 2 nanoparticles with different shell thickness can be controlled by a simple coating method. The two-dimensional assembly films of Ag @ SiO 2 nanoparticles have significant distance dependence on the enhancement effect of the fluorescent materials. When the shell thickness (5 ± 1) nm, the fluorescence enhancement of rhodamine B by nanoparticle films reached the maximum, and the enhancement factor was about 5 times. When the thickness of the nanoparticles was greater than 22 nm, the nanoparticle films had almost no fluorescence enhancement effect on rhodamine B . Cy5 ~ goat anti-rabbit protein as a fluorescent substance, and further confirmed that the core-shell Ag @ SiO 2 assembly effective enhancement of fluorescence properties.