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目的了解目前轮状病毒在太原地区的流行现状。方法用聚丙烯酰胺凝胶电泳技术对2003年11月至2004年3月采自山西省儿童医院的204腹泻标本进行了核酸检测。结果204份标本中,检出A组轮状病毒128份,占62.7%,其中长型106份(82.8%),短型22份(17.2%);还有113份标本有另一条核酸带,其中单纯感染的有47份,占41.6%;与轮状病毒混合感染的有66份,占58.4%。这些标本经RNA酶处理后,腺病毒的检出率为12.75%。结果还显示,轮状病毒感染病例最多的月份为12月,较以往推后了一个月。发病年龄无变化,97.1%为2岁以下的婴幼儿。基因的电泳型也仅有L和S2种,而不是以往的10种。结论A组轮状病毒在太原地区流行依然严重,并存在其它腹泻病毒的流行。
Objective To understand the current status of rotavirus in Taiyuan. Methods Polyacrylamide gel electrophoresis was used to detect the DNA of 204 diarrhea samples collected from Shanxi Children’s Hospital from November 2003 to March 2004. Results In 204 samples, 128 samples of A group were detected, accounting for 62.7%, of which 106 (82.8%) were long and 22 (17.2%) were short; 113 samples had another nucleic acid band, Among them, 47 cases were purely infected, accounting for 41.6%; 66 cases were mixed with rotavirus, accounting for 58.4%. After RNAse-treated these samples, the detection rate of adenovirus was 12.75%. The results also showed that rotavirus infection in most cases of the month of December, pushed back more than a month. No change in the age of onset, 97.1% of infants under 2 years of age. The electrophoretic type of gene is also only L and S2 species, rather than the previous 10 species. Conclusion A group of rotavirus epidemic in Taiyuan is still serious, and there is the prevalence of other diarrhea virus.