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目的建立鉴定心肌梗死的红外光谱法,探讨傅里叶红外光谱面扫描技术鉴别早期心肌缺血和陈旧性心肌梗死的可行性。方法利用傅里叶变换显微红外光谱面扫描成像技术对1例心肌梗死组织样品进行逐点扫描,观测细胞酰胺A带、CH3反对称伸缩、胶原蛋白三螺旋结构及核酸和磷酸脂质PO2反收缩振动频率的光谱分布情况,绘制梗死组织的红外光谱病理图像。结果酰胺A带及胶原蛋白三螺旋结构的高吸收区、CH3反对称伸缩及核酸和磷酸脂质PO2反收缩振动的低吸收区与梗死区符合。结论傅里叶红外光谱及面扫描技术可鉴定心肌梗死病理改变,酰胺A带和胶原蛋白三螺旋结构的红外光谱图改变可特征性的判别早期心肌缺血和陈旧性心肌梗死。
Objective To establish a method of infrared spectroscopy to identify myocardial infarction and explore the feasibility of using Fourier transform infrared spectroscopy to identify early myocardial ischemia and old myocardial infarction. Methods One-point myocardial infarction tissue samples were scanned point by point by Fourier transform infrared spectroscopy (FTIR). The changes of amide amide A band, CH3 antisymmetric stretching, collagen triple helix structure and nucleic acid and phospholipid PO2 Shrinkage vibration frequency spectral distribution, the infra red tissue pathological images of infarction. Results The high absorbance region of amide A band and collagen triple helix structure, CH3 antisymmetric stretching and low absorption region of anti-contractile vibration of nucleic acid and phospholipid PO2 were consistent with infarct region. Conclusion Fourier transform infrared spectroscopy and surface scan can identify the pathological changes of myocardial infarction. The changes of the infrared spectra of amide A band and collagen triple helix can characteristically distinguish early myocardial ischemia from old myocardial infarction.